Like most monasteries in Meteora and elsewhere, Roussanou had several dependencies, called metochia. A metochi can be a church, a house, or a piece of land or any other asset that belongs to a monastery and is located outside its main grounds.
The monastery’s oldest dependency is in Koperna, now known as the village of Avra in Kalambaka, which the Roussanou monks bought from the Turks in 1547. Before that, it belonged to the Meteora monastery of the Pantokrator. Two years later, in January 1576, the Ecumenical Patriarch Jeremias II confirmed in a sigillum the purchase and possession of the metochion of Avra by the flourishing and prosperous monastery of Roussanou. The original sigillum has survived and is kept in the National Library of Greece.
On 22 December 1994, the late Bishop of Stagoi and Meteora Seraphim gave the ownership of two old Meteora monydria (small monasteries) to Roussanou and entrusted it with their renovation and restoration: these being the Monastery of the Holy Apostles of Agia and the hermitage known as the Prison of the Monks (Holy Mother of God of the Prisons) or Oglas.
According to the sigillum issued by the Ecumenical Patriarch Timothy II (1614)—a summary of which was published by the Hellenist Russian archimandrite Porfyrius Uspensky—the neighbouring monydria of the Holy Archangels and Kallistratou were considered dependencies of Roussanou. The Cell of the Apostle Paul was also considered a Roussanou dependency, as noted in a summary of an epistle known as the “Testament of the stylites of the kellion of Roussanou” (1544-45) that has not survived.
In 1996, the monastery’s community acquired a dependency in Theopetra with the encouragement of the late Bishop of Stagoi and Meteora Seraphim, and the support of the Roussanou nun Veroniki and the devout benefactor Sofia Prifti. Here the nuns have established gardens and orchards in order to supply the monastery’s needs. In 2005, the nuns also set up chandlery and incense workshops at the same dependency. In 2016, they built a chapel dedicated to St Tryphon, the protector of farmers and vine growers, in the grounds of the Theopetra dependency.